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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
Published on: May 22, 2012
Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides
J F Sällström1, I Zehbe, M Alemi
1Department of Pathology, University Hospital, Uppsala, Sweden.
Anticancer Research
|July 1, 1993
Abstract:
False positivity is reported of in situ PCR reactions in a direct incorporation assay with digoxigenin-labelled dUTP. It is recommended that in situ hybridization with specific labelled probe replaces the direct incorporation method for the detection of in situ PCR amplicon.
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