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Chemiluminescence from bamboo shoot cut

H Totsune1, M Nakano, H Inaba

  • 1Bio-Photon Project, Research Development Corporation of Japan, Kojinnkai Central Hospital, Miyagi.

Biochemical and Biophysical Research Communications
|August 16, 1993
PubMed
Summary

Bamboo shoots emit light due to chemiluminescence, a phenomenon involving the enzymatic oxidation of tyrosine and bityrosine by peroxidase. This natural light emission is similar to that observed in horseradish systems.

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Area of Science:

  • Biochemistry
  • Plant Science
  • Chemiluminescence Studies

Background:

  • Bamboo shoots exhibit a weak light emission when cut.
  • This phenomenon, known as chemiluminescence, suggests an underlying biochemical process.
  • Previous studies have documented chemiluminescence in other biological systems.

Purpose of the Study:

  • To investigate the source of chemiluminescence in bamboo shoots.
  • To identify the specific compounds and enzymes involved in this light emission.
  • To compare the bamboo shoot chemiluminescence system with known systems like horseradish peroxidase.

Main Methods:

  • Utilized a two-dimensional imaging system to visualize light emission from cut bamboo shoots.
  • Performed water extraction of bamboo shoot components.

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  • Analyzed the extract for amino acids, specifically tyrosine, and enzymes like peroxidase.
  • Reconstituted a system with bamboo shoot peroxidase, hydrogen peroxide (H2O2), and tyrosine to study light emission characteristics.
  • Measured emission spectra using spectrophotometry.

    • Main Results:

    • Weak light emission was observed from cut bamboo shoots.
    • Bamboo shoot extract contained tyrosine, bityrosine, and peroxidase.
    • The bamboo shoot peroxidase-H2O2-tyrosine system produced weak light emission.
    • Emission maxima were observed at 490nm, 530nm, and longer wavelengths.
    • These spectral characteristics were identical to the horseradish peroxidase-H2O2 system.

    Conclusions:

    • Chemiluminescence from cut bamboo shoots likely originates from excited species.
    • These excited species are generated by the enzymatic oxidation of tyrosine and bityrosine.
    • The bamboo shoot chemiluminescence mechanism shares similarities with the well-characterized horseradish peroxidase system.