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Updated: Oct 12, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Free radical formation in isolated murine keratinocytes treated with organic peroxides and its modulation by
1Department of Chemistry, University of York, UK.
Abstract:
Electron paramagnetic resonance spin-trapping has been used to study the production of free radicals from tert-butyl hydroperoxide, tert-butyl peroxybenzoate, cumene hydroperoxide and ethyl hydroperoxide in isolated murine keratinocytes. Free radical species could be trapped from keratinocytes treated with all peroxides, with radicals produced from both one-electron oxidative and reductive pathways. The hindered phenolic antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), which are known to inhibit peroxide-induced tumour promotion in vivo, decreased the amount of radical adduct production at a concentration of 10 mM, with BHA being significantly more effective than BHT. That all the peroxides in this study produced free radicals in keratinocytes, and that BHA and BHT decreased the amounts of radicals trapped, suggests that free radical production by organic peroxide compounds is involved in their in vivo tumour-promoting activity.
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