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Related Experiment Videos

Adsorption losses from urine-based cannabinoid calibrators during routine use

J A Blanc1, V A Manneh, R Ernst

  • 1Development Department, Syva Co., San Jose, CA 95161-9013.

Clinical Chemistry
|August 1, 1993
PubMed
Summary

Cannabis metabolite delta 9-THC adsorbs to surfaces, causing inaccurate drug test calibrators. Proper pipette use and minimizing surface-to-volume ratios in analyzer cups significantly reduce this analyte loss.

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Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry

Background:

  • 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (delta 9-THC) is the primary urine metabolite of cannabis.
  • Delta 9-THC is crucial for calibrating cannabinoid immunoassays.
  • The hydrophobic nature of delta 9-THC leads to adsorption onto solid surfaces, compromising assay accuracy.

Purpose of the Study:

  • To quantify cannabinoid loss from calibrator solutions due to handling.
  • To identify specific handling techniques contributing to analyte loss.
  • To develop strategies for minimizing delta 9-THC adsorption during testing.

Main Methods:

  • Quantification of cannabinoid loss using immunoassay techniques.
  • Utilizing radioactive tracers to measure analyte loss.
  • Analyzing the impact of different pipette types on adsorption.

Related Experiment Videos

  • Assessing the effect of surface-to-volume ratio in analyzer cups.

    • Main Results:

    • Significant delta 9-THC losses were observed during calibrator handling.
    • Analyte loss is influenced by the type of pipette used for solution transfer.
    • The surface-to-volume ratio of the calibrator solution in the analyzer cup affects adsorption.
    • Losses were confirmed through both immunoassay and radioactive tracer methods.

    Conclusions:

    • Handling techniques are a primary cause of analyte loss in delta 9-THC calibrators.
    • Employing specific pipettes and optimizing surface-to-volume ratios can minimize adsorption.
    • Implementing suggested handling procedures enhances the accuracy of cannabinoid immunoassays.