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[Establishment and characterization of a new human testicular germ cell tumor cell line (TCam-2)]
Y Mizuno1, A Gotoh, S Kamidono
1Department of Urology, Kobe University School of Medicine.
Abstract:
A new human testicular cancer cell line (TCam-2) was established. The original material of TCam-2 was a primary lesion of a left testicular seminoma (typical pure type seminoma) from a 35 aged male patient. TCam-2 produced neither AFP nor beta-HCG, It showed strong immunoreactivities for 5G9 (anti testicular cancer MoAb), 4B3 (anti PTHrP MoAb) and PALP (placental alkaline phosphatase). The chromosomal analysis revealed 92 modal number and loss of Y chromosome. Histochemical, morphological and chromosomal analysis supported that TCam-2 is from classical seminoma. TCam-2 was transplanted subcutaneously to the back of 6 weeks old scid mice (CB-17 strain), and grew a classical seminomatous tissue.