[Change in calcium transport as a factor in causing dysfunction of myocardial mitochondria during hypoxia]

During normoxic incubation of isolated myocardial mitochondria, there were marked increases in mitochondrial calcium when free calcium concentration in the incubation medium was enhanced. The increases were concomitant with rises increase in the rate of state 4 respiration. Moreover, there is a positive correlation between the mitochondrial calcium and the rate of state 4 respiration. During hypoxic incubation, when the calcium concentration of the incubation medium was enhanced, there was no significant increase in mitochondrial calcium and the rises in the rate of state 4 respiration were much lower than the rises during normoxia. Hypoxic incubation in medium of low calcium concentration (pCa 8.0) resulted in slight rises in rate of state 4 respiration. The above results suggest that in myocytes, the mitochondrial dysfunction under hypoxia may be caused indirectly by changes in cytoplasm rather than directly by hypoxia. It seems that the change of cellular free calcium concentration is an important factor in the mitochondrial dysfunction.