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Updated: Aug 6, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Sequence analysis of cystine-containing peptides in the Na+, K(+)-ATPase alpha subunit
N M Gevondyan1, V S Gevondyan, N N Modyanov
1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Abstract:
Tryptic digest of the pig kidney Na+, K(+)-ATPase was subjected to HPLC for separating water-soluble fragments. Using ammetric titration with silver nitrate, disulfide-containing peptides were identified and demonstrated to contain s-s bonds with differential sensitivity to reduction. The amino acid sequences of cystine-containing peptides were determined by chemical modification of cysteine residues during the sequence analysis. Among the cystine-containing peptides, three fragments of the alpha-subunit polypeptide chain were identified: Cys452-Lys461, Ile507-Lys519, Val545-Phe558.
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