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Related Experiment Videos

Insertion sequence elements in Bacillus thuringiensis subsp. darmstadiensis

M Ryan1, J D Johnson, L A Bulla

  • 1Department of Molecular Biology, University of Wyoming, Laramie 82071.

Canadian Journal of Microbiology
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

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Two novel insertion sequence variants, IS231G and IS231H, were identified in Bacillus thuringiensis, exhibiting tandem repeats. These elements correlate with insect toxicity and are found on plasmids and chromosomal DNA.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Bacillus thuringiensis (Bt) is a bacterium known for its insecticidal properties.
  • Bt subspecies are often classified by their toxicity spectrum, targeting either lepidopteran or dipteran insects.
  • Insertion sequences (IS elements) are mobile genetic elements that can influence bacterial genome evolution and gene expression.

Purpose of the Study:

  • To characterize novel insertion sequence variants (IS231G and IS231H) from Bacillus thuringiensis subsp. darmstadiensis 73-E-10-2 (BTD2).
  • To investigate the genomic location and evolutionary relationships of these IS231 elements.
  • To explore the correlation between IS231 element distribution and the toxicity spectrum of the host bacterium.

Main Methods:

  • DNA sequence analysis of isolated IS231 variants.

Related Experiment Videos

  • Southern blot hybridization to determine the location of IS elements on plasmids and chromosomal DNA.
  • Comparative analysis of IS231 sequences with known IS elements from other Bt strains.

    • Main Results:

    • Two new IS231 variants, IS231G and IS231H, were identified and found to be encoded as direct tandem repeats, a previously unreported arrangement for IS231 elements.
    • These elements are closely related to other IS231 family members found in Bt strains with varying insecticidal activities.
    • IS231 elements were detected on both plasmids (55- and 34-MDa) and chromosomal DNA of BTD2.
    • BTD2 chromosomal DNA also hybridized with IS240, an unrelated IS element, indicating the presence of diverse IS elements within this strain.

    Conclusions:

    • The discovery of tandem IS231 repeats provides new insights into the organization and potential mobility of these elements.
    • The observed correlation between IS231 sequence relatedness and host toxicity spectrum suggests a role in the evolution of Bt insecticidal properties.
    • BTD2 harbors a diverse array of IS elements, including those previously associated with different Bt subspecies, highlighting horizontal gene transfer and genomic plasticity.