Identification of novel singly spliced pX mRNA transcripts common to all human T-cell leukemia virus type 1-related...

Human T-cell leukemia virus type 1 (HTLV-1) and its related viruses, that is, human T-cell leukemia virus type 2 (HTLV-2), simian T-cell leukemia virus (STLV), and bovine leukemia virus (BLV), encode a doubly spliced pX mRNA transcript in addition to the singly spliced env and unspliced gag-pol mRNAs common to the prototypic simple retroviruses, such as murine and avian leukemia viruses. In HTLV-1-infected cells, we recently identified the novel singly spliced pX mRNA responsible for expressing the smaller rex gene product, p21X. In the present study we demonstrate that the novel singly spliced pX mRNA is also expressed in cells infected with HTLV-2, STLV, and BLV, respectively. This finding indicates that all members of the HTLV-1-related virus group have the common ability to express the singly spliced pX mRNA. This novel mRNA in the HTLV-1-related virus group may be analogous to the two-exon nef specific mRNA in human immunodeficiency virus type 1.