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Epstein-Barr virus (EBV) gene expression in EBV-positive peripheral T-cell lymphomas
C L Chen1, R H Sadler, D M Walling
1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill 27599-7295.
Journal of Virology
|October 1, 1993
Summary
Epstein-Barr virus (EBV) DNA is found in clonal T-cell lymphomas. EBV gene expression in these lymphomas is distinct from other lymphoma types, resembling that of nasopharyngeal carcinomas.
Area of Science:
- Oncology
- Virology
- Immunology
Background:
- Epstein-Barr virus (EBV) is associated with various lymphoid malignancies.
- The role and expression patterns of EBV in peripheral T-cell lymphomas (PTCLs) require further elucidation.
Purpose of the Study:
- To investigate the clonality and viral gene expression patterns of EBV in PTCLs.
- To compare the EBV expression profile in PTCLs with other EBV-associated malignancies.
Main Methods:
- Analysis of EBV DNA termini to assess clonality.
- Detection of viral transcripts (mRNAs) using molecular techniques.
- Immunofluorescence for protein detection (LMP1, EBNA2).
Main Results:
- EBV infection in PTCLs was determined to be clonal and nonpermissive.
- Processed mRNAs for EBNA1, LMP1, LMP2, and BamHI A were detected in all EBV-positive PTCLs.
- LMP1 protein was detected in one case, while EBNA2 protein and mRNA were consistently absent.
Conclusions:
- EBV-infected PTCLs represent clonal expansions of a single infected cell.
- The EBV gene expression profile in PTCLs is unique, differing from Burkitt's lymphoma and post-transplant lymphoproliferative disorders.
- The observed EBV expression pattern in PTCLs is similar to that found in nasopharyngeal carcinomas.