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[Nucleotide sequence and genome structure of mink enteritis virus]
Yi Chuan Xue Bao = Acta Genetica Sinica
|January 1, 1993
Summary
This study details the mink enteritis virus (MEV) genome sequence, revealing high similarity to canine and feline parvoviruses, with key differences in the 5' non-coding region. This provides crucial insights into parvovirus evolution and structure.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Context:
- Mink enteritis virus (MEV) is a significant pathogen in mustelids.
- Understanding MEV's genetic makeup is crucial for disease control and comparative virology.
- Parvoviruses share common genomic structures and evolutionary pathways.
Purpose:
- To report the complete nucleotide sequence of the cloned mink enteritis virus (MEV) RF DNA.
- To analyze the 5' and 3' end structures of the MEV genome.
- To compare the MEV genome sequence with related parvoviruses, canine parvovirus (CPV) and feline parvovirus (FPV).
Summary:
- The MEV genome is approximately 5064 nucleotides long, featuring three 51 nt repeated sequences in its 5' non-coding region.
- MEV exhibits extremely high nucleotide sequence homology (99.1%–99.9%) with CPV and FPV in the structural gene region, but notable divergence in the 5' non-coding area.
- The MEV genome structure mirrors CPV and FPV, containing two large open reading frames encoding proteins of 668 and 722 amino acids, with promoters at map units 37 and 39, a poly A site at map unit 97, and spliced mRNAs for NS2, VP1, and VP2.
Impact:
- Provides a foundational genomic resource for MEV research.
- Facilitates comparative genomic studies within the Parvoviridae family.
- Enhances understanding of parvovirus evolution, host adaptation, and potential for cross-species transmission.