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A novel primary Ca(2+)-transport system from Saccharomyces cerevisiae
L A Okorokov1, W Tanner, L Lehle
1Institute of Biochemistry and Physiology, Academy of Sciences, Puschino, Russia.
European Journal of Biochemistry
|September 1, 1993
Summary
Researchers discovered a new calcium (Ca2+) transport system in yeast membranes. This ATP-dependent system is distinct from plasma membrane pumps and is localized to the Golgi complex.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Calcium ions (Ca2+) are crucial for various cellular processes.
- Understanding Ca2+ transport mechanisms is vital for cellular homeostasis.
- Saccharomyces cerevisiae serves as a model organism for studying eukaryotic cell functions.
Purpose of the Study:
- To identify and characterize a novel Ca2+ transport system in Saccharomyces cerevisiae.
- To determine the biochemical properties and cellular localization of this transport system.
Main Methods:
- Ca2+ uptake assays using isolated yeast membranes.
- Nucleotide dependency and inhibitor sensitivity studies.
- Localization studies using cell fractionation and marker enzyme analysis.
Main Results:
- A novel ATP-dependent Ca2+ transport system was identified.
- Transport activity was inhibited by sodium vanadate but not by other tested inhibitors.
- The Ca2+ transporter was localized to the yeast Golgi complex, co-fractionating with GDPase activity.
Conclusions:
- The identified Ca2+ transport system is an endomembrane-type ATPase, distinct from plasma membrane Ca2+-ATPases.
- This finding provides new insights into calcium regulation within the yeast endomembrane system.
- The Golgi-localized transporter plays a role in intracellular calcium trafficking.