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[Use of microwaves for enhancing or restoring antigens before immunohistochemical staining]
A J Balaton1, F Ochando, M H Painchaud
1Service d'Anatomie et de Cytologie Pathologiques, Hôpital Saint Joseph, Paris.
Annales De Pathologie
|January 1, 1993
Abstract:
In the proposed protocol, the slides are deparaffinized, rehydrated, placed in 0.01 M, pH 6 citrate buffer and irradiated 3 x 5 minutes in a microwave oven (power output = 650 Watts) before immunostaining. This enables a positive staining for some antibodies and an enhancement of staining intensity for most antibodies.