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Algorithms for detecting antibodies to HIV-1: results from a rural Ugandan cohort
A J Nunn1, B Biryahwaho, R G Downing
1Medical Research Council Programme on AIDS in Uganda, Uganda Virus Research Institute, Entebbe.
Objective:
To evaluate an algorithm using two enzyme immunoassays (EIA) for anti-HIV-1 antibodies in a rural African population and to assess alternative simplified algorithms.
Methods:
Sera obtained from 7895 individuals in a rural population survey were tested using an algorithm based on two different EIA systems: Recombigen HIV-1 EIA and Wellcozyme HIV-1 Recombinant. Alternative algorithms were assessed using negative or confirmed positive sera.
Results:
None of the 227 sera classified as unequivocably negative by the two assays were positive by Western blot. Of 192 sera unequivocably positive by both assays, four were seronegative by Western blot. The possibility of technical error cannot be ruled out in three of these. One of the alternative algorithms assessed classified all borderline or discordant assay results as negative had a specificity of 100% and a sensitivity of 98.4%. The cost of this algorithm is one-third that of the conventional algorithm.
Conclusions:
Our evaluation suggests that high specificity and sensitivity can be obtained without using Western blot and at a considerable reduction in cost.