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Related Experiment Videos

Developmental expression of the IGF-II/mannose 6-phosphate receptor

P Nissley1, W Kiess, M Sklar

  • 1Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

Molecular Reproduction and Development
|August 1, 1993
PubMed
Summary

The insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) is highly expressed in fetal rat tissues and declines after birth. IGF-II/M6PR mRNA levels correlate with protein levels, indicating its role in development.

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Area of Science:

  • Developmental Biology
  • Molecular Endocrinology
  • Cell Biology

Background:

  • The insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) plays crucial roles in cellular processes.
  • Developmental regulation of IGF-II/M6PR is suggested by its presence in fetal serum.

Purpose of the Study:

  • To investigate the developmental regulation of IGF-II/M6PR expression in rat tissues.
  • To determine the relationship between IGF-II/M6PR mRNA and protein levels during development.

Main Methods:

  • Quantitative immunoblotting was used to measure IGF-II/M6PR protein levels in serum and tissues.
  • Northern blotting and solution hybridization/RNase protection assays were employed to assess IGF-II/M6PR mRNA expression.

Main Results:

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  • Circulating IGF-II/M6PR was significantly higher in fetal and early postnatal sera, decreasing sharply thereafter.
  • IGF-II/M6PR protein levels were high in fetal tissues, with a distinct rank order (heart > placenta > lung = intestine > muscle = kidney > liver > brain).
  • Fetal tissues exhibited higher IGF-II/M6PR mRNA concentrations compared to postnatal tissues, mirroring protein expression patterns.

Conclusions:

  • IGF-II/M6PR expression is developmentally regulated in rats, with peak levels during embryonic and fetal development.
  • IGF-II/M6PR mRNA levels are a key determinant of receptor protein abundance in most tissues.
  • The prominent expression of IGF-II/M6PR during mammalian embryonic/fetal periods highlights its potential specialized functions distinct from non-mammalian vertebrates.