Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments

M Hawkins¹, B Pope, S K Maciver

¹MRC Laboratory of Molecular Biology, Cambridge, England.

|September 28, 1993

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Understanding pregnant women's health literacy strengths and challenges: a cluster-based exploration in the Health Literacy in Pregnancy (HeLP) study.

Public health·2025

Same author

Employing innovation to enhance the safety and reliability of restorative surgical techniques for patients with familial adenomatous polyposis at a national referral centre.

Techniques in coloproctology·2024

Same author

SCOPE 2 - Still Answering the Unanswered Questions in Oesophageal Radiotherapy? SCOPE 2: a Randomised Phase II/III Trial to Study Radiotherapy Dose Escalation in Patients with Oesophageal Cancer Treated with Definitive Chemoradiation with an Embedded Phase II Trial for Patients with a Poor Early Response using Positron Emission Tomography/Computed Tomography.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022

Same author

Risk of colorectal adenomas and cancer in monoallelic carriers of MUTYH pathogenic variants: a single-centre experience.

International journal of colorectal disease·2021

Same author

Corrigendum to "Considerations for the Treatment of Oesophageal Cancer with Radiotherapy During the COVID-19 Pandemic" [Clin Oncol 32 (2020) 354-357].

Clinical oncology (Royal College of Radiologists (Great Britain))·2021

Same author

Conflicts and contradictions in current skin cancer screening guidelines.

Current dermatology reports·2021

Human actin depolymerizing factor (ADF) binds to actin monomers and filaments. Its activity, including severing actin filaments, is highly dependent on pH levels.

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Actin depolymerizing factor (ADF) is an actin-binding protein abundant in neuronal cells.
ADF plays a crucial role in regulating actin dynamics within cells.

Purpose of the Study:

To clone and characterize human ADF.
To investigate the biochemical properties and actin-binding behavior of human ADF.
To determine the effect of pH on ADF's interaction with actin.

Main Methods:

Cloning of human ADF.
Expression of human ADF in Escherichia coli.
Biochemical assays to assess binding to G-actin and F-actin.
pH-dependent activity studies.

Related Experiment Videos

Main Results:

Human ADF sequence is identical to porcine destrin.
Recombinant human ADF mimics native ADF's behavior.
ADF sequesters G-actin at pH 8, preventing polymerization.
ADF severs F-actin in a calcium-insensitive manner, with low efficiency at pH 8.
ADF binds cooperatively to F-actin below pH 7, leading to rapid severing and depolymerization upon pH increase.

Conclusions:

Human ADF is functionally equivalent to porcine destrin.
ADF's interaction with actin is pH-dependent, influencing its monomer-sequestering and filament-severing activities.
These findings highlight ADF's role in dynamic actin regulation, particularly in neuronal cells.