Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium imaging

A Trautmann1, Y P Tan

  • 1Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France.

Blood Cells
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

This review covers key considerations, limitations, artifacts, and optimal applications for calcium (Ca2+) imaging systems. Understanding these factors is crucial for effective biological research using Ca2+ imaging techniques.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-ideal penetration of shaped charge jet into spaced plate based on drift velocity and gap effects.

Scientific reports·2026
Same author

Fungal Planet description sheets: 1781-1866.

Persoonia·2025
Same author

Fungal Planet description sheets: 1697-1780.

Fungal systematics and evolution·2025
Same author

Fungal Planet description sheets: 1614-1696.

Fungal systematics and evolution·2024
Same author

Fungal Planet description sheets: 1550-1613.

Persoonia·2024
Same author

Fungal Planet description sheets: 1478-1549.

Persoonia·2024
Same journal

In vitro and in vivo studies of stromal niches.

Blood cells·1994
Same journal

Differential manipulation of normal and chronic myeloid leukemia stem cell proliferation in vitro.

Blood cells·1994
Same journal

Enrichment for primitive hemopoietic progenitors of marrow cells from 5-fluorouracil-treated mice and normal mice.

Blood cells·1994
Same journal

Protein tyrosine phosphorylation in the regulation of hematopoiesis by receptors of the cytokine-receptor superfamily.

Blood cells·1994
Same journal

Lysis of blood platelets by human osteosarcoma cells in vitro.

Blood cells·1994
Same journal

Development of hemopoietic bone marrow within the ectopic bone induced by bone morphogenetic protein.

Blood cells·1994
See all related articles

Area of Science:

  • Biophysics
  • Cell Biology
  • Neuroscience

Background:

  • Calcium ions (Ca2+) are critical intracellular messengers involved in numerous cellular processes.
  • Accurate measurement of Ca2+ dynamics is essential for understanding cell signaling and function.
  • Calcium imaging techniques provide a powerful tool for visualizing these dynamic changes in living cells and tissues.

Purpose of the Study:

  • To delineate the essential components and considerations for establishing a Ca2+ imaging system.
  • To identify and discuss the inherent limitations and potential challenges associated with Ca2+ imaging.
  • To explore common artifacts encountered in Ca2+ imaging and their impact on data interpretation.

Main Methods:

  • This review synthesizes existing knowledge on Ca2+ imaging system design and performance.

Related Experiment Videos

  • It analyzes the technical constraints and sources of error in various Ca2+ imaging modalities.
  • The review discusses the practical aspects of applying Ca2+ imaging to diverse biological questions.

    • Main Results:

    • Key elements for Ca2+ imaging systems include indicator choice, microscopy hardware, and data acquisition parameters.
    • Limitations involve signal-to-noise ratio, phototoxicity, and temporal/spatial resolution.
    • Common artifacts include uneven dye loading, photobleaching, and out-of-focus light.

    Conclusions:

    • Ca2+ imaging systems require careful optimization of hardware and reagents for specific applications.
    • Awareness of system limitations and artifacts is paramount for reliable data acquisition and interpretation.
    • Selecting the appropriate Ca2+ imaging approach enhances its utility across various biological research fields.