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Related Experiment Videos

Testing deficiency paternity cases with a Y-linked tetranucleotide repeat polymorphism

F R Santos1, J T Epplen, S D Pena

  • 1Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

EXS
|January 1, 1993
PubMed
Summary
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Y-linked genetic markers, like Y-27H39, offer superior exclusion power in paternity testing, especially when the father is unavailable. This study confirms their utility in deficiency cases involving deceased fathers and male relatives.

Area of Science:

  • Forensic Genetics
  • Human Genetics
  • Molecular Biology

Background:

  • Paternity testing typically uses autosomal DNA markers.
  • Y-linked polymorphisms offer unique advantages in paternity cases due to direct paternal inheritance.
  • Challenges arise in paternity testing when the alleged father is deceased or unavailable.

Purpose of the Study:

  • To evaluate the effectiveness of the Y-linked tetranucleotide repeat locus Y-27H39 in paternity deficiency cases.
  • To assess the utility of Y-27H39 when the alleged father is deceased and only male relatives are available for testing.

Main Methods:

  • Analysis of 41 families with a deceased alleged father using multilocal and unilocal DNA fingerprinting.
  • Specific evaluation of the Y-linked locus Y-27H39 in cases with male probands and available male relatives.

Related Experiment Videos

  • Calculation of paternity index and exclusion analysis.

    • Main Results:

    • Y-27H39 significantly contributed to the paternity index in 13 cases where paternity was included.
    • Exclusion by Y-27H39 was observed in one out of three cases where fingerprinting probes also excluded paternity.
    • The Y-linked probe was applicable in approximately 40% of the tested cases.

    Conclusions:

    • The Y-linked locus Y-27H39 is a valuable tool for deficiency paternity cases, particularly when autosomal markers have limited power.
    • It is especially useful when only one male relative of a deceased father is available for testing.
    • While effective, Y-linked probes have application limitations, being usable in about 40% of cases.