Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Forensic DNA typing by the solid-phase minisequencing method

A C Syvänen1, A Sajantila, M Lukka

  • 1Department of Human Molecular Genetics, National Public Health Institute, Helsinki, Finland.

EXS
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

European Council of Legal Medicine (ECLM) on-site inspection forms for forensic pathology, anthropology, odontology, genetics, entomology and toxicology for forensic and medico-legal scene and corpse investigation: the Parma form.

International journal of legal medicine·2022
Same author

Global genetic variation of select opiate metabolism genes in self-reported healthy individuals.

The pharmacogenomics journal·2017
Same author

Temporal variation in coat colour (genotypes) supports major changes in the Nordic cattle population after Iron Age.

Animal genetics·2016
Same author

Bones hold the key to DNA virus history and epidemiology.

Scientific reports·2015
Same author

Post-mortem analysis of lactate concentration in diabetics and metformin poisonings.

International journal of legal medicine·2015
Same author

Cause and manner of death and phase of the blood alcohol curve.

Forensic science international·2014

This study presents a DNA-typing method using solid-phase minisequencing to identify single nucleotide variations for reliable forensic identification. A novel approach for rapid allele frequency determination using pooled DNA samples is also introduced.

Area of Science:

  • Molecular Biology
  • Forensic Science
  • Genetics

Background:

  • DNA typing is crucial for individual identification in forensic science.
  • Existing methods for DNA analysis can be time-consuming and resource-intensive.

Purpose of the Study:

  • To develop an efficient and reliable DNA-typing method for forensic identification.
  • To introduce a novel approach for rapid allele frequency determination.

Main Methods:

  • Utilized solid-phase minisequencing to detect a panel of 12 biallelic markers.
  • Employed Polymerase Chain Reaction (PCR) to amplify DNA fragments.
  • Introduced quantitative analysis of pooled DNA samples for allele frequency determination.

Main Results:

Related Experiment Videos

  • The selected panel of 12 markers proved effective for individual identification.
  • The minisequencing method demonstrated efficiency and reliability in DNA typing.
  • The novel approach allows for rapid determination of allele frequencies.

Conclusions:

  • Solid-phase minisequencing is a robust method for forensic DNA typing.
  • The developed method offers an efficient and reliable solution for individual identification.
  • The quantitative analysis of pooled DNA samples provides a rapid alternative for allele frequency studies.