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Forensic DNA typing by the solid-phase minisequencing method
A C Syvänen1, A Sajantila, M Lukka
1Department of Human Molecular Genetics, National Public Health Institute, Helsinki, Finland.
EXS
|January 1, 1993
Abstract:
We describe a method for DNA-typing, in which a panel of biallelic markers are detected by the solid-phase minisequencing method (Syvänen et al., 1990). This method identifies single nucleotide variations in DNA fragments amplified by the PCR. Determination of the panel of 12 markers selected in this study proved to be an efficient and reliable method for forensic identification of individuals. We also introduce a novel approach for rapid determination of allele frequencies by quantitative analysis of pooled DNA samples.