Related Experiment Videos
Immunodetection of low concentrations of ovalbumin in cryoprocessed quail oviduct cells by semi-automatic
C Quintana1, C López-Iglesias, N Bonnet
1U303 INSERM, La Darse, Villefranche-sur-mer, France.
Biology of the Cell
|January 1, 1993
Abstract:
In a previous paper (Quintana et al, Biol Cel 72, (1991) 167-180) we reported the anti-ovalbumin antibody immunolabeling in the cytoplasm of tubular gland cells from cryofixed, cryosubstituted and acrylic-resin embedded quail oviduct. To confirm these preliminary visual observations, we have carried out a semi-automatic quantitative study of immunogold labeling. The quantitative results confirm the previous data. In addition, we found a significant higher immunolabeling with low temperature Lowicryl K11M embedding procedure as compared with high temperature LR White embedding.