Related Experiment Videos
Method for 3D volumetric analysis of intranuclear fluorescence distribution in confocal microscopy
Abstract:
The introduction of the confocal laser scanning microscope makes it possible to acquire fluorescent specimens in 3D. We present basic image processing tools to enhance the data and to quantitate the morphology and topography of the intravolume elements. The tools were applied to the description of the spatial distribution of DNA replication sites in mammalian cell nuclei as an example.