Related Experiment Videos
Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ
S K Bohlander1, R Espinosa, A A Fernald
1Section of Hematology/Oncology, University of Chicago, Illinois 60637.
Cytogenetics and Cell Genetics
|January 1, 1994
Abstract:
We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulse-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.