Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protooncogene junB as a target for activin actions

M Hashimoto1, D Gaddy-Kurten, W Vale

  • 1Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037-1099.

Endocrinology
|November 1, 1993
PubMed
Summary

Activin rapidly increases junB messenger RNA (mRNA) levels in various cells. This induction is transcription-dependent and occurs independently of protein synthesis, suggesting junB is a key event in activin signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A rat resistance exercise regimen attenuates losses of musculoskeletal mass during hindlimb suspension.

Acta physiologica Scandinavica·2002
Same author

Inhibin suppresses and activin stimulates osteoblastogenesis and osteoclastogenesis in murine bone marrow cultures.

Endocrinology·2001
Same author

Matrix metalloproteinases in multiple myeloma.

Leukemia & lymphoma·2000
Same author

Activin increases phosphorylation and decreases stability of the transcription factor Pit-1 in MtTW15 somatotrope cells.

The Journal of biological chemistry·1995
Same author

Inactivation of activin-dependent transcription by kinase-deficient activin receptors.

Endocrinology·1995
Same author

Activins and the receptor serine kinase superfamily.

Recent progress in hormone research·1995

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Gene Expression

Background:

  • Activin, a transforming growth factor-beta peptide, regulates cell growth and differentiation.
  • Identifying immediate early genes offers insight into nuclear events driving activin-regulated cellular changes.

Purpose of the Study:

  • To identify immediate early genes involved in activin-mediated cellular responses.
  • To investigate the role of junB gene expression in activin signaling pathways.

Main Methods:

  • Treatment of human K562 and rat PC12 cells with activin.
  • Analysis of junB, c-jun, and c-fos messenger RNA (mRNA) levels.
  • Assessment of junB induction in the presence of 12-O-tetradecanoyl phorbol-13-acetate and forskolin.

Related Experiment Videos

Main Results:

  • Activin induced a rapid, transcription-dependent increase in junB mRNA in both cell types within 1 hour.
  • Neither c-jun nor c-fos mRNA levels were significantly affected by activin treatment.
  • Activin-induced junB mRNA expression was independent of protein synthesis and protein kinase C (PKC) and protein kinase A (PKA) pathways.

Conclusions:

  • Activin selectively induces junB mRNA, suggesting its critical role in mediating activin's effects.
  • The induction of junB represents a common nuclear event in diverse activin-responsive tissues.
  • JunB gene induction by activin is a rapid, transcription-dependent process independent of major signaling kinases.