Nerve-specific marker proteins as indicators of organic solvent neurotoxicity

Effects of chronic exposure to n-hexane and toluene on some nerve-specific marker proteins in rat central nervous system (CNS) and peripheral nervous system (PNS) were assessed and compared. The rats were exposed to 2000 ppm n-hexane, 12 hr/day, 6 days/week, for 24 weeks, and to 1000 ppm toluene, 8 hr/day, 6 days/week, for 16 weeks. The level of neuron-specific enolase (NSE), creatine kinase-B (CK-B), and beta-S100 protein in cortex, cerebellum, spinal cord, and proximal and distal sciatic nerve was determined by enzyme immunoassay method. In n-hexane-exposed rats, the level of NSE, CK-B, and beta-S100 decreased significantly in the distal segment of the sciatic nerve, while the marker proteins in CNS and proximal sciatic nerve remained unchanged. In contrast, chronic exposure to toluene mostly affected these marker proteins in CNS tissues, displaying the increase of NSE, CK-B, beta-S100 in cerebellum, as well as the increase of beta-S100 in spinal cord. No quantitative changes of the three proteins in distal sciatic nerve were observed after exposure to toluene. n-Hexane-induced peripheral distal neuropathy and toluene-induced brain gliosis appeared to be responsible for this different pattern of biochemical changes. The present study suggests the usefulness of using these nerve-specific marker proteins to assess the solvent-related CNS and PNS neurotoxicity.