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Mouse intestinal epithelial cells express the self superantigen Mls1a
D Kaiserlian1, K Vidal, H R MacDonald
1Unité d'Immunologie et Stratégie Vaccinale, Institut Pasteur de Lyon, France.
European Journal of Immunology
|October 1, 1993
Summary
Normal enterocytes present the self superantigen Mls1a via major histocompatibility complex class II, influencing T cell receptor V beta repertoire selection in the gut.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- Intestinal epithelial cells (IECs) can present exogenous antigens to CD4+ T cells.
- The role of IECs in presenting endogenous antigens and influencing T cell repertoire selection is less understood.
Purpose of the Study:
- To investigate the capacity of normal enterocytes to present the self superantigen Mls1a.
- To elucidate the mechanism of Mls1a presentation by enterocytes.
- To explore the implications for extrathymic T cell selection.
Main Methods:
- Utilized Mls1a and Mls1b mouse strains.
- Assessed interleukin-2 production in V beta 6+ T cell hybridomas.
- Employed antibody inhibition assays, including monoclonal antibodies against Orf protein.
- Investigated major histocompatibility complex class II (MHC-II) dependency.
Main Results:
- Enterocytes from Mls1a strains stimulated interleukin-2 production in Mls1a-specific T cell hybridomas.
- Mls determinant presentation by enterocytes is dependent on MHC-II.
- Monoclonal antibodies against Orf protein inhibited T cell activation, linking Mtv-7 to Mls1a expression on enterocytes.
Conclusions:
- Mls determinants are expressed on normal enterocytes.
- Enterocytes play a role in the extrathymic selection of the T cell receptor V beta repertoire in intraepithelial lymphocytes.