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Expression in E. coli and purification of a chimeric p22-NS3 recombinant antigen of hepatitis C virus (HCV)
S Osborne1, E Cecconato, S Griva
1R&D Diagnostic Division, SORIN Biomedica S.p.A., Saluggia, Italy.
FEBS Letters
|June 21, 1993
Abstract:
A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a starting point for the design of an HCV single all-encompassing antigen for a blood screening assay.