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Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I
K L Armour1, W J Harris, P R Tempest
1Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, Scotland.
Gene
|September 15, 1993
Abstract:
We have cloned and sequenced the human cardiac troponin I (cTnI)-encoding cDNA with the aim of expressing the cDNA in Escherichia coli. The cDNA was successfully expressed as a fusion product with beta-galactosidase and as an unfused protein. Both polypeptides were recognised by an anti-human cTnI antibody.