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Sequence of manganese superoxide dismutase-encoding cDNAs from multiple mouse organs
Y Sun1, G Hegamyer, N H Colburn
1Cell Biology Section, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.
Gene
|September 15, 1993
Summary
Researchers identified three sequence differences in mouse manganese superoxide dismutase (MnSOD) genes across various organs. These findings are crucial for verifying wild-type sequences and detecting potential mutations.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Manganese superoxide dismutase (MnSOD) is a critical antioxidant enzyme.
- Previous studies reported a specific MnSOD cDNA sequence for mouse placenta.
Purpose of the Study:
- To investigate sequence variations in mouse MnSOD cDNAs across different organs.
- To compare the MnSOD sequence from multiple organs with the previously reported placental sequence.
Main Methods:
- Reverse transcriptase-polymerase chain reaction (RT-PCR) was employed.
- Direct sequencing of amplified cDNA was performed on RNA from BALB/c mouse organs and placenta.
Main Results:
- Three sequence differences were identified in MnSOD cDNAs from liver, kidney, brain, spleen, and heart compared to the published placental sequence.
- These differences included specific amino acid substitutions (Val(-7)-->Gly and Met(114)-->Val) and a deletion in the 3' untranslated region (G746 deletion).
- Analysis of BALB/c mouse placenta RNA revealed that its MnSOD sequence matched that found in other organs.
Conclusions:
- The previously published mouse placenta MnSOD sequence appears to be non-wild-type.
- The sequence identified in multiple organs represents the wild-type MnSOD sequence.
- These findings are valuable for accurate wild-type sequence verification and the detection of point mutations in mouse MnSOD.