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Updated: Aug 7, 2026

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
Published on: January 9, 2013
Shortcomings of some currently available DNA probes for malaria detection
1Division of Life Sciences, Institute of Fundamental Studies, Kandy, Sri Lanka.
Abstract:
A non-radioactive DNA probe based-method for detecting malaria will greatly aid epidemiological studies. Using putative Plasmodium falciparum and P. vivax-specific 18S ribosomal RNA directed oligonucleotides, different enzymatic and chemiluminescent detection methods were attempted without success. The sensitivity of the corresponding 32P-labelled probes was found to be inadequate. A published procedure based on chemiluminescent detection of repetitive DNA sequences of P. falciparum was found to be adequately sensitive but lacking in specificity.
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