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Motile sperm in human testis biopsy specimens
W W Jow1, J Steckel, P N Schlegel
1James Buchanan Brady Foundation, Department of Urology, New York Hospital-Cornell Medical Center 10021.
Journal of Andrology
|May 1, 1993
Summary
Intraoperative testis biopsy wet preps can identify sperm, aiding diagnosis. Motile sperm in wet preps strongly indicate reproductive tract obstruction, justifying immediate reconstructive surgery.
Area of Science:
- Reproductive Medicine
- Andrology
- Surgical Pathology
Background:
- Infertile men often undergo testis biopsies to diagnose spermatogenesis issues.
- Intraoperative assessment of testis biopsy material is crucial for timely surgical decisions.
Purpose of the Study:
- To evaluate the diagnostic utility of intraoperative wet preparation cytology of testis biopsy specimens.
- To correlate wet prep findings with the presence of reproductive tract obstruction and spermatogenesis status.
Main Methods:
- Prospective study of 62 infertile men undergoing testis biopsy.
- 100 intraoperative wet preps of fresh testis tissue analyzed for sperm presence and motility.
- Comparison with permanent histological sections for spermatogenesis and obstruction assessment.
Main Results:
- Sperm with tails identified in 62% of wet preps; 18% contained motile sperm.
- Motile sperm were exclusively found in obstructed testes with complete spermatogenesis.
- Wet prep findings showed high positive predictive values for obstruction (100% for motile sperm) and spermatogenesis.
Conclusions:
- Intraoperative wet preparation of testis biopsy is a valuable tool in andrology.
- Presence of motile sperm in wet preps is a strong indicator of reproductive tract obstruction.
- This rapid assessment can guide immediate surgical exploration and reconstructive procedures.