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CO2 production in cell-free extracts of fission yeast detects cell cycle changes
B Novak1, A Sveiczer, J M Mitchison
1Institute of Cell, Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Scotland.
Journal of Cell Science
|June 1, 1993
Abstract:
CO2 production was followed by manometry in starved cell-free extracts of fission yeast stimulated by unstarved cell-free extracts from a synchronous culture. The degree of stimulus, measured by the lag time in CO2 production, varied markedly during the cell cycle, with a maximum for cells at about mitosis and a minimum for septated cells. Similar differences in lag time were found with unstarved extracts of cdc13.117 grown at 37 degrees C and 35 degrees C.