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Thiol and disulfide determination by free zone capillary electrophoresis
M Cappiello1, A Del Corso, M Camici
1Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
Journal of Biochemical and Biophysical Methods
|July 1, 1993
Abstract:
A rapid, sensitive and simple method for the determination of reduced and oxidized glutathione, cysteine, cystine, cysteamine, cystamine and their respective mixed disulfides is described. The compounds were separated and identified in a single step by capillary zone electrophoresis. The method was used to follow the thiol-disulfide interconversion and to measure glutathione levels in lens extracts.