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A modified method for obtaining large amounts of high titer polyclonal ascites fluid
S K Ou1, J M Hwang, P H Patterson
1California Institute of Technology, Pasadena 91125.
Journal of Immunological Methods
|September 27, 1993
Summary
Researchers developed a modified method to produce large quantities of polyclonal ascites fluid (AF) from mice, even with limited antigen amounts. This technique yields high-titer AF, crucial for antibody research and diagnostics.
Area of Science:
- Immunology
- Biotechnology
- Biochemistry
Background:
- Limited antigen availability poses challenges in generating sufficient polyclonal antisera.
- Traditional methods using small rodents often result in low yields of antisera.
Purpose of the Study:
- To develop an improved method for generating high-titer polyclonal ascites fluid (AF) when antigen quantities are restricted.
Main Methods:
- A modified ascites fluid generation protocol was designed.
- Mice were injected with 1-100 micrograms of protein antigen and appropriate adjuvant.
- Compatible sarcoma cells were administered via intraperitoneal injection.
Main Results:
- High serum titers (1:1000-1:20,000) of polyclonal AF were achieved.
- Each mouse yielded 13-19 ml of high-titer AF.
- The method proved effective across nine different antigens.
Conclusions:
- The modified method efficiently produces substantial volumes of high-titer polyclonal AF from limited antigen inputs.
- This technique offers a viable solution for antibody production in research and diagnostics.