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Identification of the gene encoding the major capsid protein of fish lymphocystis disease virus
1Institut für Medizinische Virologie, Universitt Heidelberg, Germany.
Abstract:
The gene encoding the major capsid protein (MCP) of fish lymphocystis disease virus (flounder isolate; FLCDV-f) has been identified by PCR using oligonucleotide primers corresponding to different regions of the MCP of Tipula iridescent virus (TIV), iridescent virus 22 (IV22) and Chilo iridescent virus (CIV). DNA fragments of 0.4 kbp, 0.5 kbp and 0.27 kbp in size were amplified using oligonucleotide primers corresponding to amino acids (aa) 146 to 153 (primer 1) and 274 to 268 (primer 6), or aa 146 to 153 (primer 1) and 313 to 304 (primer 8), or aa 304 to 312 (primer 7) and 385 to 381 (primer 9) of the MCP of TIV, respectively. The PCR products were used as hybridization probes for screening the gene library of FLCDV-f. The MCP gene of FLCDV-f(1377 bp; 459 aa; 51.4K) was identified within the DNA sequence of the EcoRI FLCDV-f DNA fragment C (11.2 kbp; 0.611 to 0.718 map units). A high degree of aa sequence identity/similarity was detected between the MCP of FLCDV-f and TIV (50.3%/33.8%), IV22 (49.1%/34.2%). CIV (53%/29.5%) and African swine fever virus (16%/38.1%).
Insights
The major capsid protein (MCP) gene of fish lymphocystis disease virus (FLCDV-f) was identified using PCR and hybridization techniques. This fish virus MCP shows significant sequence similarity to other iridoviruses.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Lymphocystis disease virus (FLCDV) causes significant economic losses in aquaculture.
- The major capsid protein (MCP) is a key structural component of iridoviruses.
Purpose of the Study:
- To identify and characterize the gene encoding the major capsid protein (MCP) of fish lymphocystis disease virus (FLCDV-f).
- To analyze the sequence similarity of FLCDV-f MCP with MCPs from other iridoviruses.
Main Methods:
- Polymerase chain reaction (PCR) was employed using primers derived from related iridovirus MCP sequences.
- PCR products served as hybridization probes to screen a FLCDV-f gene library.
- The identified MCP gene sequence was analyzed for amino acid identity and similarity.
Main Results:
- The complete MCP gene of FLCDV-f (1377 bp, encoding 459 amino acids) was successfully identified.
- FLCDV-f MCP exhibited significant amino acid sequence identity/similarity to MCPs of Tipula iridescent virus (TIV), iridescent virus 22 (IV22), and Chilo iridescent virus (CIV).
- Moderate similarity was also observed with African swine fever virus.
Conclusions:
- The study successfully identified the MCP gene of FLCDV-f, confirming its iridovirus classification.
- Comparative sequence analysis provides insights into the evolutionary relationships among iridovirus MCPs.
- This finding contributes to understanding the molecular biology and taxonomy of fish lymphocystis disease virus.