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Procoagulant activities expressed by peripheral blood mononuclear cells
P B Tracy1, R A Robinson, L A Worfolk
1Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
Methods in Enzymology
|January 1, 1993
Summary
Mononuclear cells, especially monocytes, initiate blood clotting via receptor-mediated assembly of enzymes on their surface. This cell surface provides a unique platform for studying coagulation regulation and thrombin production.
Area of Science:
- Cellular biology
- Hematology
- Biochemistry
Background:
- Mononuclear cells, particularly monocytes, play a crucial role in the procoagulant response.
- Previous research indicates monocytes provide a membrane surface essential for coagulation complex assembly and thrombin production in vivo.
Purpose of the Study:
- To support the concept of regulated binding site-mediated assembly of proteolytic activities on mononuclear cell surfaces.
- To highlight monocytes as a key site for investigating the regulation of cell surface coagulant reactions.
Main Methods:
- Analysis of combined data from multiple laboratories.
- Investigating receptor-mediated events and product channeling in coagulation reactions.
Main Results:
- Mononuclear cells, especially monocytes, elicit a procoagulant response through regulated binding site-mediated assembly of proteolytic activities.
- Monocytes provide the necessary membrane surface for the assembly and function of coagulation complexes required for thrombin production.
Conclusions:
- Monocytes are central to cell surface-mediated coagulation.
- Monocytes offer a unique model for studying the regulation of coagulant reactions via receptor-mediated events and substrate channeling.