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Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing
B A Larder1, A Kohli, P Kellam
1Antiviral Therapeutic Research Unit, Wellcome Foundation Limited, Beckenham, Kent, UK.
Nature
|October 14, 1993
Abstract:
A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.