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Related Experiment Videos

Three-dimensional structure of a membrane-containing virus

A M Paredes1, D T Brown, R Rothnagel

  • 1Cell Research Institute, University of Texas at Austin 78713.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1993
PubMed
Summary

The Sindbis virus structure reveals two icosahedral protein shells and cellular membrane. This finding clarifies alphavirus assembly and stability, impacting viral morphogenesis research.

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Area of Science:

  • Virology
  • Structural Biology
  • Biophysics

Background:

  • Sindbis virus is a model alphavirus.
  • Understanding viral structure is key to understanding viral assembly and function.

Purpose of the Study:

  • To determine the three-dimensional structure of the Sindbis virus.
  • To elucidate the structural organization and protein interactions within the virion.

Main Methods:

  • Electron cryomicroscopy was used to image intact Sindbis virus particles.
  • Three-dimensional reconstruction techniques were applied to the electron microscopy data.

Main Results:

  • The Sindbis virus virion exhibits two icosahedral protein shells (T=4 lattice).
  • A cellular membrane bilayer separates the viral protein shells.

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  • Structural evidence of interaction between glycoprotein and nucleocapsid protein across the membrane was observed.

    • Conclusions:

    • The determined structure provides insights into alphavirus morphogenesis.
    • Specific protein associations across the membrane are crucial for virion stability.
    • A potential mechanism for Sindbis virus assembly has been proposed.