Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MDM2 gene amplification in metastatic osteosarcoma

M Ladanyi1, C Cha, R Lewis

  • 1Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

Cancer Research
|January 1, 1993
PubMed
Summary

MDM2 gene amplification is linked to osteosarcoma progression. This amplification was found more often in metastatic or recurrent tumors than in primary ones, suggesting a role in tumor spread.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring natural language processing as a tool to improve in obstetric documentation and care.

International journal of obstetric anesthesia·2025
Same author

Ventromedial prefrontal cortex activation and neurofeedback modulation during episodic future thinking for individuals with suicidal thoughts and behaviors.

Behaviour research and therapy·2024
Same author

Improving Survival with Medicaid Expansion in Early Hepatocellular Carcinoma: A Step in the Right Direction.

Annals of surgical oncology·2023
Same author

Identification of Two Forms of Human Plasma Renalase, and Their Association With All-Cause Mortality.

Kidney international reports·2020
Same author

Capacity building for global nursing leaders: challenges and experiences.

International nursing review·2016
Same author

Tongue Cancer.

Postgraduate medicine·2016

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The MDM2 gene, a regulator of p53 protein function, is frequently amplified in sarcomas.
  • MDM2 gene amplification may lead to functional inactivation of the p53 protein, a crucial tumor suppressor.
  • Osteosarcoma is a high-grade bone cancer where MDM2 amplification is being investigated.

Purpose of the Study:

  • To investigate the clinical and pathological correlations of MDM2 gene amplification in high-grade osteosarcoma.
  • To determine if MDM2 gene amplification is associated with tumor progression and metastasis in osteosarcoma.

Main Methods:

  • Southern blot analysis was used to detect MDM2 gene amplification in 28 osteosarcoma specimens from 26 patients.
  • Polymerase chain reaction (PCR) was employed to isolate MDM2 complementary DNA probes for the analysis.

Related Experiment Videos

  • Specimens included primary, metastatic, and locally recurrent high-grade osteosarcomas.

    • Main Results:

    • MDM2 gene amplification was detected in 14% (4/28) of the osteosarcoma specimens.
    • Amplification was observed in 3 metastatic and 1 local recurrence specimen; none were found in primary osteosarcomas.
    • MDM2 gene amplification was significantly more frequent in metastatic or recurrent osteosarcomas compared to primary ones (P = 0.02).

    Conclusions:

    • MDM2 gene amplification is associated with tumor progression and metastasis in osteosarcoma.
    • The findings suggest MDM2 amplification may play a role in the aggressiveness of osteosarcoma.
    • Further research is needed to explore the clinicopathological significance of MDM2 gene amplification in osteosarcoma.