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Detection of Francisella tularensis in blood by polymerase chain reaction
G W Long1, J J Oprandy, R B Narayanan
1Center for Immunization Research, Johns Hopkins University, Baltimore, Maryland 21205.
Journal of Clinical Microbiology
|January 1, 1993
Abstract:
We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.