Ectoplasmic ("junctional") specializations in Sertoli cells of the rooster and turtle: evolutionary implications

Ectoplasmic specializations are complex actin-containing structures found at certain sites of intercellular attachment in Sertoli cells. Current evidence indicates that these structures are a form of actin-associated adhesion junction. In the turtle (Pseudemys scripta) and rooster (Gallus domesticus) ectoplasmic specializations are known to occur adjacent to sites of attachment to elongate spermatids and are characterized by a layer of "loosely" cross-linked actin filaments that lies next to the plasma membrane. In the turtle, a cistern of endoplasmic reticulum is associated with the cytoplasmic face of the filament layer. We have found that, in fixed frozen sections of turtle and rooster testes, immunological probes for myosin II react with epithelial regions that also stain with probes for filamentous actin and that are known to be sites at which ectoplasmic specializations occur. Furthermore, when exposed to standard contraction buffers, the diameters of glycerinated ectoplasmic specializations of the turtle are statistically smaller than those of the same structures exposed to control buffers. We interpret these smaller diameters as being produced by the contraction of actin bundles within the ectoplasmic specializations. Our results indicate that ectoplasmic specializations in the rooster and turtle, unlike those in mammals, possess contractile properties. We speculate that ectoplasmic specializations in eutherian mammals may have evolved from actin-associated adhesion junctions in which the actin bundles were initially contractile and from which myosin II was secondarily lost.