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Human platelets secrete chemotactic activity for eosinophils

J A Burgers1, R C Schweizer, L Koenderman

  • 1Department of Hematology, University Hospital Utrecht, The Netherlands.

Blood
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

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Platelets release factors that attract eosinophils and increase their calcium levels. These platelet-derived factors may play a role in allergic and inflammatory conditions.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Platelets are known to release various factors upon activation by thrombin.
  • Eosinophils are key cells in allergic and inflammatory responses.
  • The interaction between platelets and eosinophils is not fully understood.

Purpose of the Study:

  • To investigate the role of platelet-derived factors in eosinophil chemotaxis and calcium mobilization.
  • To identify the specific platelet components responsible for these effects.

Main Methods:

  • Platelet activation assays using thrombin.
  • Measurement of eosinophil chemotaxis.
  • Assessment of cytosolic calcium ([Ca2+]i) in eosinophils.
  • Studies using platelets from patients with Storage-Pool Deficiency and degranulated platelets.

Related Experiment Videos

  • Testing the effects of specific platelet-secreted factors like adenosine triphosphate (ATP), adenosine diphosphate (ADP), and platelet factor 4.

    • Main Results:

    • Thrombin-stimulated platelets release factors that induce eosinophil chemotaxis and increase cytosolic calcium ([Ca2+]i).
    • These factors originate from platelet dense- and alpha-granules.
    • Platelets from Storage-Pool Deficiency patients showed reduced chemotactic activity.
    • Completely degranulated platelets lost their ability to affect eosinophils.
    • Adenosine triphosphate (ATP) induced chemotaxis and increased [Ca2+]i, but other factors in platelet releasates contributed to a stronger effect than ATP alone.

    Conclusions:

    • Platelets secrete factors that activate eosinophils, influencing their migration and intracellular calcium.
    • These platelet-derived factors, including ATP and others, may contribute to inflammatory and allergic processes.
    • Understanding this platelet-eosinophil crosstalk is crucial for developing targeted therapies.