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Related Experiment Videos

Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen

R S Israeli1, C T Powell, W R Fair

  • 1Urologic Oncology Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

Cancer Research
|January 15, 1993
PubMed
Summary

Researchers cloned the gene for prostate-specific membrane antigen (PSMA), a protein found exclusively in prostate cells. This integral membrane protein shows potential for targeted cancer therapies and imaging.

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Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • A novel M(r) 100,000 prostate-specific membrane glycoprotein (PSM) was identified using the monoclonal antibody 7E11-C5.
  • PSM is exclusively expressed in normal and neoplastic prostate cells and their metastases.

Purpose of the Study:

  • To report the molecular cloning of a full-length complementary DNA (cDNA) encoding the PSM antigen.
  • To analyze the cDNA sequence and the deduced polypeptide structure.

Main Methods:

  • Polymerase chain reaction (PCR) using degenerate oligonucleotide primers.
  • Screening of a human LNCaP cell line complementary DNA library.
  • DNA sequence analysis.

Main Results:

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  • A full-length 2.65-kilobase cDNA encoding PSM was successfully cloned.
  • A 54% homology was found between a portion of the PSM coding region (nucleotides 1250-1700) and human transferrin receptor mRNA.
  • The deduced polypeptide contains a putative transmembrane domain, allowing differentiation of intra- and extracellular portions.

Conclusions:

  • PSM, as an integral membrane protein, differs from secreted prostate proteins like prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP).
  • PSM's membrane-bound nature makes it a promising target for developing advanced imaging and cytotoxic targeting modalities in prostate cancer treatment.