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Tetranectin: a novel secretory protein from human monocytes
H Nielsen1, I Clemmensen, A Kharazmi
1Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Scandinavian Journal of Immunology
|January 1, 1993
Summary
Tetranectin, a human plasma protein, is secreted by monocytes and macrophages. This secreted tetranectin enhances the spontaneous migration of these immune cells.
Area of Science:
- Immunology
- Cell Biology
- Protein Biochemistry
Background:
- Tetranectin is a human plasma protein found in various secretory cells, including neutrophils.
- Its presence and function in monocytes and macrophages were previously uncharacterized.
Purpose of the Study:
- To investigate the presence and secretion of tetranectin in human monocytes and macrophages.
- To determine the functional effects of tetranectin on monocyte and neutrophil behavior.
Main Methods:
- Immunoblotting was used to identify tetranectin released by monocytes and macrophages.
- Monocyte migration assays and oxidative metabolism assays were performed.
- Neutrophil functions were also assessed.
Main Results:
- Human monocytes and macrophages secrete tetranectin (3-18 ng/h/10(6) cells) upon adherence or weak stimulation.
- Purified tetranectin significantly enhanced monocyte spontaneous migration.
- Tetranectin did not affect monocyte chemotactic or chemiluminescence responses, nor neutrophil functions.
Conclusions:
- Tetranectin is secreted by human mononuclear phagocytes (monocytes and macrophages) following weak stimulation.
- Secreted tetranectin promotes the spontaneous migration of monocytes, suggesting a role in immune cell trafficking.