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Membrane-incorporated surrogate receptors increase antigen presentation by resting B cells, but not by LPS-activated

C M Gontijo1, G Möller

  • 1Department of Biochemistry and Immunology, Federal University of Minas Gerais, Brazil.

Scandinavian Journal of Immunology
|January 1, 1993
PubMed

Palmitate-conjugated monoclonal antibodies specific to ovalbumin were inserted into the cell membrane of normal resting B cells and LPS-activated blasts. These two decorated B cells were tested for their ability to act as antigen-presenting cells for ovalbumin-specific I-Ad-restricted T-cell hybridomas. It was found that the antibody-decorated resting B cells presented antigen more efficiently than non-decorated controls. However, no increment was observed when decorated LPS blasts were compared with non-decorated blasts. This is explained by the fact that the inserted antibodies quickly disappeared from the cell membrane of LPS blasts, while they were retained for a long period in the membrane of resting B cells.

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