Cryosurgery

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Updated: Oct 13, 2026

Flash Freezing and Cryosectioning E12.5 Mouse Brain
28:32

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Published on: May 27, 2007

G F Graham1

  • 1Department of Dermatology, University of North Carolina School of Medicine, Chapel Hill.

Clinics in Plastic Surgery
|January 1, 1993
PubMed

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...