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Published on: December 31, 2014
c-fos gene expression in cell revertants from a transformed to a pseudonormal phenotype
Y V Lavrovsky1, F P Svinarchuk, V A Lavrovsky
1Institute of Bioorganic Chemistry, Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk.
Abstract:
c-fos gene expression in two types of mouse sarcoma cells of spontaneous origin and in revertants to pseudonormal phenotype has been investigated. In the latter cells the content of c-fos mRNA is similar to that in normal fibroblasts. Activity of transcription factors interacting with the regulatory elements, SRE, DSE and TRE, in the c-fos promoter do not correlate with the c-fos mRNA concentration. However, experiments with cells transformed with the indicator plasmid, fos-CAT, showed that the 600 bp c-fos promoter region provides the chloramphenicol acetyltransferase activity correlating with c-fos mRNA expression in cell revertants to a pseudonormal phenotype.
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