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Crystallization and preliminary crystallographic characterization of the copper-containing amine oxidase from pea
V Vignevich1, D M Dooley, J M Guss
1Department of Inorganic Chemistry, University of Sydney, N.S.W., Australia.
Journal of Molecular Biology
|January 5, 1993
Abstract:
The copper-containing amine oxidase from pea seedlings has been crystallized using lithium sulfate as precipitant at pH 5.2. The unit cell is orthorhombic, space group P2(1)2(1)2(1), with dimensions a = 89.3 A, b = 113.4 A, c = 199.0 A. The mass of the asymmetric unit is 131(+/- 13) kDa, consistent with independent evidence that the molecule has two approximately 66 kDa subunits. The crystals diffract to 2.5 A in a synchrotron X-ray beam.