Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

How do gender and military sexual trauma impact PTSD symptoms in cognitive processing therapy and prolonged exposure?

Journal of psychiatric research·2020

Same author

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.

Development genes and evolution·2016

Same author

Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer.

Oncogene·2016

Same author

Microarray coupled to quantitative RT-PCR analysis of androgen-regulated genes in human LNCaP prostate cancer cells.

Oncogene·2009

Same author

Capsaicin-sensitive primary sensory neurons in the mouse express N-Acyl phosphatidylethanolamine phospholipase D.

Neuroscience·2009

Same author

Inhibiting estrogen responses in breast cancer cells using a fusion protein encoding estrogen receptor-alpha and the transcriptional repressor PLZF.

Gene therapy·2005

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

In vitro regulation of HGF-SF expression by epithelial-mesenchymal interactions

T Kamalati¹, R F Brooks, N Holder

¹Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.

|January 1, 1993

Summary

Researchers developed cell culture models to study molecules regulating cell behavior, including proliferation, differentiation, and movement. These models aid understanding of development, wound healing, and tumor metastasis, focusing on HGF-SF expression regulation.

More Related Videos

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

Related Experiment Videos

Last Updated: Jul 9, 2026

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

Area of Science:

Cell Biology
Molecular Biology
Developmental Biology

Background:

Cellular processes like proliferation and differentiation are fundamental to growth, development, and disease.
Understanding cell-cell interactions is key to characterizing molecules that influence cell behavior.
Dysregulation of these processes is implicated in wound healing, regeneration, tumor invasion, and metastasis.

Purpose of the Study:

To present novel cell culture models for studying phenomena affecting cell behavior.
To apply these models to investigate the regulation of Hepatocyte Growth Factor-Scatter Factor (HGF-SF) expression.
To identify molecules that modulate cell proliferation, differentiation, and movement through gene expression changes.

Main Methods:

Development of specialized cell culture systems.
Analysis of gene expression patterns.
Functional assays to assess cell proliferation, differentiation, and migration.
Application of models to study specific molecular pathways, such as HGF-SF regulation.

Main Results:

Established cell culture models provide a platform for studying complex cellular behaviors.
Demonstrated the utility of these models in investigating the regulation of HGF-SF.
Identified key parameters influencing cell proliferation, differentiation, and movement.

Conclusions:

The developed cell culture models are effective tools for dissecting molecular mechanisms controlling cell behavior.
These models facilitate research into fundamental biological processes and disease pathologies.
Further application of these models can advance understanding of HGF-SF and related signaling pathways.