A simplified method for generating step-wise deletions using PCR
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1Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Gene
|January 15, 1993
Abstract:
A simple and general method is described for the generation of ordered deletions for DNA sequencing. Nicked plasmids, the substrates for step-wise digestion by exonuclease III, are obtained after the self-ligation of PCR products with phosphorylated and non-phosphorylated primers and plasmid DNAs as template. The method is suitable for use with any plasmid vector and for generation of deletion clones with deletions in both possible directions.
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