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Genome analysis of adenovirus 4 isolated over a six year period
R J Cooper1, A S Bailey, R Killough
1North Manchester Regional Virus Laboratory, Booth Hall Children's Hospital, England.
Abstract:
Genome analysis was carried out on 74 adenovirus 4 (Ad4) isolates from patients in Manchester between 1984 and 1989. Most of the isolates were associated with conjunctivitis. Of the 74 isolates studied, 51 were Ad4a and 10 were Ad4p (the prototype strain). The remaining isolates consisted of two new genome types we have designated Ad4a2 (10 isolates) and Ad4a3 (3 isolates). Most of the genome types co-circulated during the period of study. The Bst E II and Xho I restriction maps of the new variants are presented and compared with those of Ad4p. We are unable to associate genome types with particular clinical presentations.
Insights
Genome analysis of 74 adenovirus 4 (Ad4) isolates revealed two new genome types, Ad4a2 and Ad4a3, co-circulating with existing types between 1984-1989. No specific genome type was linked to clinical presentations like conjunctivitis.
Area of Science:
- Virology
- Molecular Biology
- Epidemiology
Background:
- Adenovirus 4 (Ad4) is a common cause of conjunctivitis.
- Understanding Ad4 genome diversity is crucial for tracking its spread and evolution.
Purpose of the Study:
- To analyze the genomic diversity of Ad4 isolates from Manchester (1984-1989).
- To identify and characterize novel Ad4 genome types.
- To compare restriction maps of new variants with the prototype strain.
Main Methods:
- Genome analysis of 74 Ad4 isolates.
- Restriction enzyme digestion using Bst E II and Xho I.
- Comparison of restriction maps.
Main Results:
- 51 isolates were Ad4a, 10 were Ad4p (prototype).
- Two new genome types, Ad4a2 (10 isolates) and Ad4a3 (3 isolates), were identified.
- Multiple Ad4 genome types co-circulated during the study period.
- Restriction maps for Bst E II and Xho I were generated for new variants.
Conclusions:
- The study identified novel Ad4 genome types, expanding the known diversity of Ad4.
- No correlation was found between specific Ad4 genome types and clinical presentations.